Reporter

Part:BBa_K592020:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)


PFixK2-lambda C1-B0034-amilCP blue light sensor output


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Q04510 inverter contains B0015 double terminator which gives unspecific primer binding of VR. Touchdown PCR was required to amplify the sequence for screening.


Source

FixK2 promoter: Bradyrhizibium japonicum amilCP: Acropora millepora

References